10X MOPS Buffer for EZ Rich Defined E. coli Growth Medium


To purchase, please click here to login.

SKU Product Manufacturer Price: Quantity
D-0508-10 D-0508-10K - 0.132M K2PO4 ***** 1
  • Reproducible Results – Using a defined medium in which all of the ingredients are known vs. an undefined media containing yeast extract or casein hydrolysate, enables tight replication of results.
  • Lower Your Regulatory Burden - Because Korz does not contain any animal-derived components, no certificate of origin is required.
  • Reduced Risk – The elimination of undefined components significantly reduces the risk of process related impurities associated with complex biologicals.

Background

Using synthetic biology methods, the Escherichia coli K-12 genome was reduced by making a series of planned, precise deletions. The multiple-deletion series (MDS™) strains (1), with genome reduction of up to 15%, were designed by identifying non-essential genes and sequences for elimination, including recombinogenic or mobile DNA and cryptic virulence genes, while preserving robust growth on rich and minimal media for either protein or plasmid production. Genome reduction also led to unanticipated beneficial properties, including high electroporation efficiency and accurate propagation of recombinant genes and plasmids that are unstable in other strains. Subsequent deletions and introduction of useful alleles produce strains suitable for many molecular biology applications.

MOPS- 3-(N-morpholino) propanesulfonic acid. MOPS (pKa = 7.20) is an excellent buffer for many biological systems at near-neutral pH, MOPS Minimal is designed for culturing E. coli and works very well with our Clean Genome® strains.

Figures

Specifications

Kit Components
10X MOPS, 100mL
0.132M K2PO4, 10mL

Quality Control
The media is confirmed for growth of Clean Genome® Strains using liquid culture and confirmed aseptic by no growth in liquid media and agar plates.

Storage Conditions
Store 10X MOPS reagent at -20°C. Store 0.132M K2PO4 at room temperature.

Patents & Disclaimers

Products are sold for non-commercial use only, under Scarab Genomics limited use label license: Limited Label Use.

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications.

Clean Genome® is a registered trademark of Scarab Genomics, LLC.