Ultra HD Minimal Feed Media with 62.8% Glucose, 1X, Sterile


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SKU Product Manufacturer Price: Quantity
S300-F-1L S300-F-1L - Ultra HD Minimal Feed Media with 62.8% Glucose, 1X, Sterile ***** 1
  • Rich Media Results in a Minimal Medium – Scarab systematically determined the metabolic needs of the Clean Genome® E. coli strains in fed batch fermentation for a variety of protein and DNA targets. Ultra HD Minimal Feed Media together with the optimized metabolism of the Clean Genome® strains enable ultra high densities up to 300 OD600 resulting in proteins yields of >40 g/L of test protein and 700 – 1000 mg/L plasmid.
  • Reproducible Results – Using a defined medium in which all of the ingredients are known vs. an undefined media containing yeast extract or casein hydrolysate, enables tight replication of results.
  • Lower Your Regulatory Burden - Because Ultra HD Minimal Feed Media does not contain any animal-derived components, no certificate of origin is required.
  • Reduced Risk – The elimination of undefined components significantly reduces the risk of process related impurities associated with complex biologicals.
  • Save Time - Glucose included for easy use.

Background

Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Ultra HD Minimal Feed Media formulation resulted from Scarab Genomics’ systematic determination of the metabolic needs of the Clean Genome® E. coli strains in fed batch fermentation for a variety of protein and DNA targets. Ultra HD Minimal Feed Media together with the optimized metabolism of the Clean Genome® strains enable ultra high densities up to 300 OD600 resulting in proteins yields of >40 g/L of test protein and 700 – 1000 mg/L plasmid in 10L scale fermentations without

The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements, and uses glucose as the carbon source. The same base medium used for the batch phase of fed-batch fermentation can be used in optimizing expression in shake flasks, thereby providing continuity between the two processes. A separate Feed Medium (Cat. No. S300-F-1L) supplies glucose, magnesium, iron, and trace elements for the feeding stage of fed-batch fermentation.

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Specifications

Kit Components
Ultra HD Minimal Feed Media with 62.8% Glucose, 1X, Sterile

Quality Control
The media is confirmed for growth of Clean Genome® Strains using liquid culture and confirmed aseptic by no growth in liquid media and agar plates.

Storage Conditions
Store at +20-25ºC and protected from light.

Patents & Disclaimers

Products are sold for non-commercial use only, under Scarab Genomics limited use label license: Limited Label Use.

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications.

Clean Genome® is a registered trademark of Scarab Genomics, LLC.