pSX2 Expression Vector


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SKU Product Manufacturer Price: Quantity
P-3330 pSX2 Expression Vector ***** 1
  • ScarabXpress®-2 Protein Expression System – Consists of the pSX2 expression vector PLUS any Clean Genome® E. coli host strain.
  • 3000-Fold Induction Range - Enables a wide induction range to optimize your target’s expression level for maximum.
  • Minimal Basal Expression – Tight regulation of transcription keep background expression low, key for target genes that deliriously affect the host or for periplasmic expressed targets.
  • Ultra High Yield – Achieve up to 36x better target yields than standard expression vectors with wild-type E. coli strains.
  • Maximum Host Compatability – pSX2 is compatible with ANY Clean Genome® E. coli strain, including our advance protease deletion strains.

Background

ScarabXpress®-2 system, our second-generation protein expression system, consists of the pSX2 expression plasmid and ANY Clean Genome® E. coli host strain. In side by side comparisons, the ScarabXpress®-2 system protein expression system gave up to 36x higher yields than standard expression vectors with wild-type E. coli strains

Figures


Figure 1. pSX2 Expression Plasmid Map.


Figure 2. 3000-Fold Induction Range. IPTG Titration of pSX2-GFP, intensity was measured, replicates were averaged and graphed in average fluorescent units.


Figure 3. ScarabXpress2 Produces ≤ 36x More Protein Than BL21(DE3).


Figure 4. More Consistent Fermentations With ScarabXpress®-2

Specifications

Kit Components
pSX2 Expression Vector, 10 μg

Quality Control
The vector is confirmed to be IS-free and for functionality.

Storage Conditions
Store at ≤ -70°C. Do not store in a frost-free freezer.

Support

Product Manuals
pSX2 Expression Vector

Patents & Disclaimers

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications.

Clean Genome® is a registered trademark of Scarab Genomics, LLC.