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10X Modified Korz Medium Kit


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SKU Product Manufacturer Price: Quantity
D-0710-100 D-0710-100K - 10X Modified Korz Medium, 100 ml ***** 1
D-0710-1L D-0710-1LK - 10X Modified Korz Medium, 1000 ml ***** 1
  • Reproducible Results – Using a defined medium in which all of the ingredients are known vs. an undefined media containing yeast extract or casein hydrolysate, enables tight replication of results.
  • Lower Your Regulatory Burden - Because Korz does not contain any animal-derived components, no certificate of origin is required.
  • Reduced Risk – The elimination of undefined components significantly reduces the risk of process related impurities associated with complex biologicals.

Background

To simplify the use of minimal medium, Scarab Genomics offers a two component kit consisting of 10X Modified Korz Medium and a separate 50X Magnesium Sulfate solution. The concentrated minimal medium and associated Magnesium Sulfate solution are diluted to create a 1X medium. A carbon source must then be added to support cell growth. We would recommend the equivalent of 0.2% glucose. The diluted 1X medium (with carbon source and appropriate antibiotics) is used for expression optimization in shake flasks. The same medium can also serves as the “batch” phase medium in fed-batch fermentations. Typically, the level of the carbon source would be adjusted to a higher level than that used in the shake flask e.g. when using glucose, the glucose level would be increased to 0.5%.

Scarab’s Clean Genome® strains were specifically designed for the production of biotherapeutic protein and DNA. The “cleanest” medium to use for biotherapeutic production is a chemically defined, minimal medium. Accordingly, Modified Korz Minimal Medium has been extensively tested with the Scarab Clean Genome® Strains to verify its ability to support cell growth and the production of recombinant protein. Korz minimal medium was originally designed for high density fed-batch fermentation of E. coli (Korz et al. 1995). The medium consists of phosphate buffer, magnesium, ferric citrate, trace elements. The user needs to supply the carbon source. The same base medium used for optimizing expression in shake flasks can also be used for fed-batch fermentation, thereby providing continuity between the two processes. In fed-batch fermentations, the same medium is simply supplemented with higher carbon source content.

Specifications

Kit Components

    D-0710-100K
    D-0710-1LK
10X Modified Korz Medium 100 ml (D-0710-100) 1000 ml (D-0710-1L)
50X Magnesium Sulfate 20 ml (D-0710-100M) 200 ml (D-0710-1LM)


Quality Control
The media is confirmed for growth of Clean Genome® Strains using liquid culture and confirmed aseptic by no growth in liquid media and agar plates.

Storage Conditions
Store the 10X Modified Korz Medium component at +4-12°C

Patents & Disclaimers

Products are sold for non-commercial use only, under Scarab Genomics limited use label license: Limited Label Use.

Scarab is providing you with this Material subject to the non-transferable right to use the subject amount of the Material for your research at your academic institution. The Recipient agrees not to sell or otherwise transfer this Material, or anything derived or produced from the Material to a third party. NO RIGHTS ARE PROVIDED TO USE THE MATERIAL OR ANYTHING DERIVED OR PRODUCED FROM THE MATERIAL FOR COMMERCIAL PURPOSES. If the Recipient makes any changes to the chromosome of the Material that results in an invention in breach of this limited license, then Scarab will have a worldwide, exclusive, royalty-free license to such invention whether patentable or not. If the Recipient is not willing to accept the terms of this limited license, Scarab is willing to accept return of this product with a full refund, minus shipping and handling costs. For information on obtaining a license to this Material for purposes other than research, please contact Scarab’s Licensing Department. Scarab Genomics’ technology is covered by U.S. Pat. No. 6,989,265 and related foreign applications.

Clean Genome® is a registered trademark of Scarab Genomics, LLC.