Long-read Sequencing = Results!

Closing Genome Assemblies via Long-read Sequencing

Scarab is looking for collaborators!

Scarab presented a poster at AGBT 2017 titled De Novo Genome Assembly for the Enterobacteriaceae Tree of Life using Long Read MinION Sequencing.

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Scarab Genomics is open for closure... genome sequencing closure! We are looking for Early Access Collaborators to perform long-read sequencing using nanopore sequencing technology. Let us combine Oxford Nanopore Technologies and Scarab’s extensive experience with DNA manipulation and handling to facilitate genome assembly and closure for you.

24 Enterobacterial Genomes Closed

Background: University of Wisconsin investigators had Roche 454 and Illumina sequence coverage for 21 of 24 microbial genomes in a project to contribute to the Tree Of Life for enterobacteria. Assembly of the sequences yielded many contigs for each genome (table). Investigators collaborated with Scarab Genomics to produce 2D long-read nanopore sequence with the goal of achieving fully closed assemblies of all 24 genomes.

Methods: DNA libraries were prepared using the recommended 2D protocol from Oxford Nanopore and sequenced on the MinION sequencing system. All MinION reads >1 kb for each genome were then assembled with the CANU assembler (Koren et at., doi: 0.1101/071282).

Results: As the table shows, Scarab’s long-read sequencing generated a single genomic contig for each of the 24 genomes.

The chart below summarizes the data from the assembly of microbial genome #10. Over half of the total >430 Mb of sequence generated originated from reads greater than 23 kb long. Reads >100kb were particularly helpful in joining any remaining separate contigs in the target genomes.

For full table details, please see case studies.

Contact Us

Let us help you close your genome assemblies! We’re recruiting collaborators to validate our technology. Contact us today to discuss early access pricing of your genome assembly.

Scarab Genomics
1202 Ann St.
Madison, WI 53713

Toll-Free (US) 888.513.7075
or 608.257.1624