Scarab Genomics is upgrading its inventory management and order processing systems. Unfortunately, during this period (Sept. 17 to Oct. 13, 2018) we are not able to accept orders. We will begin accepting orders Monday, Oct. 15, 2018. We apologize for any inconvenience. Please contact us at info@scarabgenomics.com with any questions or concerns.

Posters, Reports, and Papers

Posters

Economic QBD Production of the Conjugate Vaccine Carrier Protein, CRM197 by a Continuous Manufacturing Process Using Scarab Genomics’ Clean Genome® E. coli

Bacterial Insertion Sequence Mobilization and Transfer to Plasmids: A Common Source of Cloning Artifacts

Automated On-line Sampling and Nutrient Monitoring for Characterizing Glucose Consumption of Microbial Fermentation Cultures

Reports

Scarab Genomics Proprietary Platform for Continuous Manufacturing of Pharmaceutical Biologics Applied to CRM197

Clean Genome - A Superior Host for Unstable Plasmid DNA

Production of DNA Vaccines Free from Mobile DNA

E. coli Host Case Study: SCARABXPRESS®-1(T7lac) Yields 12X More Protein Than BL21(DE3)

Bacterial Insertion Sequence Mobilization and Transfer to Plasmids: A Common Source of Cloning Artifacts

Papers

Multiple Deletion Strain Clean Genome® E. coli

Blattner, F.R., et al., 1997. The complete sequence of Escherichia coli K-12. Science 277 (5331): 1453-1474.

Bower and Prather (2009) Engineering of bacterial strains and vectors for the production of plasmid DNA. Appl Microbio Biotechnol; 82 (April 5); 805-813.

Chacko S. Chakiath, CS & Esposito, D (2007): Improved Recombinational Stability of Lentiviral Expression Vectors Using Reduced-Genome Escherichia coli. BioTechniques 43:466-470.

Csörgo et al. (2012) Low-Mutation-Rate, Reduced-Genome Escherichia coli an Improved Host for Faithful Maintenance of Engineered Genetic Constructs Microbial Cell Factories, 11:11.

Kolisnychenko, V., et al., 2002. Engineering a reduced Escherichia coli genome. Genome Res. 12(4): 640-7.

Perna, N.T., et al., 2001. Genome sequence of enterohemorrhagic Escherichia coli O157:H7. Nature 409: 529-533.

Pósfai G, et al., (2006) Emergent Properties of Reduced-Genome Escherichia coli. Science 312:1044-6.

Sharma, S., et al., 2006. Recombinant protein production in an Escherichia coli reduced genome strain. Metabolic Engineering 9 (2007) 133-141.

Sharma, S., et al., 2007. Expression of Two Recombinant Chloramphenicol Acetyltransferase Variants in Highly Reduced Genome Escherichia coli Strains. Biotechnology and Bioengineering, Vol. 98, No. 5.

Ussery, David W., 2006. Leaner and meaner genomes in Escherichia coli. Genome Biology 2006, Volume 7, Issue 10, Article 237.

Welch, R.A., et al., 2002. Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli. Proc. Natl. Acad. Sci. USA, 99(26): 17020-17024.

Ying et al. (2013) Multilevel Comparative Analysis of the Contributions of Genome Reduction and Heat Shock to the E.coli Transcriptome. BMC Genomics, 14:25.

Insertion Elements

Gibson, D. G., et al., 2010. Creation of a bacterial cell controlled by a chemically synthesized genome. Science. Jul 2;329(5987):52-6.

Kiss, J., and Olasz, F., (1999) Formation and transposition of the covalently closed IS30 circle: the relation between tandem dimers and monomeric circles. Mol Microbiol. 34(1):37-52.

Prather, K.L.J. et al., (2006) Identification and characterization of IS1 transposition in plasmid amplification mutants of E. coli clones producing DNA vaccines. Appl Microbiol Biotechnol. 73(4):815-26.

Sekine, Y. et al., (1997) Isolation and characterization of IS1 circles. Gene. 191(2):183-90.

Shiga, Y. et al., (1999) Transposition of IS1 circles. Genes Cells. 4(10):551-61.

Turner, P.C., et al., 2012. Optical mapping and sequencing of the Escherichia coli KO11 genome reveal extensive chromosomal rearrangements, and multiple tandem copies of the Zymomonas mobilis pdc and adhB genes. J Ind Microbiol Biotechnol. Apr;39(4):629-39.

Umenhoffer et al. (2010) Reduced Evolvability of Escherichia coli MDS42, an IS-Less Cellular Chassis for Molecular and Synthetic Biology Applications. Microbial Cell Factories, 9:38.

van der Heijden et al. Transposon Leads to Contamination of Clinical pDNA Vaccine. (2013) Vaccine. Jul 11;31(32):3274-80.

Plasmid DNA

Bower and Prather (2009) Engineering of bacterial strains and vectors for the production of plasmid DNA. Appl Microbio Biotechnol; 82 (April 5); 805-813.

Chacko S. Chakiath, CS & Esposito, D (2007): Improved Recombinational Stability of Lentiviral Expression Vectors Using Reduced-Genome Escherichia coli. BioTechniques 43:466-470.

Csörgo et al. (2012) Low-Mutation-Rate, Reduced-Genome Escherichia coli an Improved Host for Faithful Maintenance of Engineered Genetic Constructs Microbial Cell Factories, 11:11.

van der Heijden et al. Transposon Leads to Contamination of Clinical pDNA Vaccine. (2013) Vaccine. Jul 11;31(32):3274-80.

Wang, Z., et al., 2004. Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation. Gene Ther. Apr;11(8):711-21.

Wild, J., Hradecna, Z., and Szybalski, W. (2002). Conditionally Amplifiable BACs: Switching from Single-Copy to High-Copy Vectors and Genomic Clones. Genome Research 12, 1434-1444.

Vaccines and Vaccine Components

Advantages of Scarab Genomics’ CRM197-A Highly Pure, Economical Carrier Protein

Bower and Prather (2009) Engineering of bacterial strains and vectors for the production of plasmid DNA. Appl Microbio Biotechnol; 82 (April 5); 805-813.

Csörgo et al. (2012) Low-Mutation-Rate, Reduced-Genome Escherichia coli an Improved Host for Faithful Maintenance of Engineered Genetic Constructs Microbial Cell Factories, 11:11.

van der Heijden et al. Transposon Leads to Contamination of Clinical pDNA Vaccine. (2013) Vaccine. Jul 11;31(32):3274-80.

Wang, Z., et al., 2004. Detection of integration of plasmid DNA into host genomic DNA following intramuscular injection and electroporation. Gene Ther. Apr;11(8):711-21.

Fermentation Media

Korz DJ, Rinas U, Hellmuth K, Sanders EA, Deckwer WD. J Biotechnol. 1995 Feb 21;39(1):59-65. Simple Fed-Batch Technique for High Cell Density Cultivation of Escherichia coli.

Protein Expression

Sharma, S., et al., 2006. Recombinant protein production in an Escherichia coli reduced genome strain. Metabolic Engineering 9 (2007) 133-141.

Sharma, S., et al., 2007. Expression of Two Recombinant Chloramphenicol Acetyltransferase Variants in Highly Reduced Genome Escherichia coli Strains. Biotechnology and Bioengineering, Vol. 98, No. 5.